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Frequently Asked Questions
Pathology FAQ
- Where is the Pathology Lab located?
The Pathology Laboratory in CCMS is located in Annenberg
Building Floor 26-Room 92.
- What are the services provided by the Pathology Lab
in CCMS?
The services provided by the Pathology Lab in CCMS are:
- necropsy and histopathology in large and small lab animals
- parasitology
- hematology
- biochemistry
- bacteriology
- serology (ELISA test in mice and rats infectious diseases)
- molecular biology-PCR for Helicobacter spp. in rodents
- Which are the rodent zoonotic diseases?
See Table 2.
- What is the sample volume required for CBC analysis,
and what is the additive used for it?
For CBC the volume required for performing the test is
20 microliters blood collected on E. D. T. A.
- How much does a CBC analysis cost?
The cost of one CBC analysis is $13.18.
- What is the sample volume required for Chemistry analysis?
For Chemistry analysis, the volume for each test required
is between 5-50 microliters depending of the panel requested.
- How much does a Chemistry analysis cost?
The cost of Chemistry test varies upon the number of the
analysis/panel. Ex. One glucose test is $5.54. If a panel
of 4 tests are requested (ALT, AST, BUN, Creatinine), the
cost is $16.16. For complete info, please call the CCMS Pathology
Laboratory at (212) 241-1696.
- What is the sample volume required for Rodent Serology
Panels?
The volume required for rodent serology panels varies
depending of the number of virus antibodies requested per
panel. At least 50 microliters of serum is necessary for a
panel of 7 viruses.
- What type of sample is required for PCR Helicobacter
detection?
For a PCR Helicobacter test, sterile collected rodent
pellets (feces) in a sterile tube are required.
- What is the IMPACT Test?
The IMPACT (Infectious, Microbe, PCR, Amplification Test)
tests biological specimens for murine pathogens such as cell
lines, tumor cells, or hybridomas before they are injected
into the rodents.
The test is performed by Radil Laboratory at the University
of Missouri.
- What is the method for collection and preparation for
testing the murine pathogens in biological specimens (culture
cells, hybridomas, and tumor cells) for the IMPACT Test?
Contact the Pathology
Laboratory Director at ext 43008 for instructions.
- How do I submit specimens for Histology processing?
The samples for Histology processing should be fixed in
Buffered Formalin 10 percent, and the volume of the fixative should
be 10 times higher than the tissue size.
Remarks:
It is advisable that the lungs be inflated with the fixative
before submerging in the fixative.
The intestinal content should be washed from the intestinal
tract before fixation.
- What is the cost of a slide of H&E staining?
One slide stained H&E costs $8.79.
- Where are the Necropsy Rooms in CCMS?
The CCMS Necropsy room is located in Annenberg Building
Room 26-93 and East Building S1-27 H.
- What tests are performed in Rodent Quarantine?
See Table 1 for the protocol for Rodent
Tests in quarantine.
- What is the duration of Rodent Quarantine?
The duration of Rodent Quarantine is 5 weeks. See Table
1 for details.
Table 1
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Test 1
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Test 2
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Test 3
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Samples from
investigator animals at arrival:
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2 weeks after
arrival: Sac 2 Sentinels
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2 wks later (4 wks after arrival):
Sac last 2 sentinels:
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Tests to perform:
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Tests to perform:
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Tests to perform:
Tape, hair, feces
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Serology: Tracking
& Tape, Hair, Feces
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Serology: Assess. & Tracking
& Tape, hair, feces,
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If Imported:
Bacteriology and add Ectro. to Serology
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If imported-Bacteriology
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Helicobacter
Testing:
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HelicobacterTesting:
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HelicobacterTesting:
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Pellets for
Helicobacter (divide in lab)
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If Negative
for Helicobacter, on the first test-
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If Negative for Helicobacter
on the first and
second tests -
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If Positive:
send to CRL for ID (hepaticus, bilis)
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collect Pellets
from investigator cages.
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collect Pellets from investigator
cages.
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(Divide in Lab)
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(Divide in Lab)
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Put 4 sentinels
in isolator
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If Positive-
Send to CRL for ID (hepaticus, bilis)
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If Positive -Send to
CRL for ID (hepaticus, bilis)
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- What is the duration of Monkey Quarantine?
The duration of Monkey Quarantine is 60 days.
- What is the protocol in monkey bite accidents?Contact the Pathology
Laboratory Director at ext 43008 for monkey bite protocol.
- What collateral laboratories are used for special or
confirmation tests?
The collateral labs used for special or confirmations
tests are:
- Charles River Lab-Wilmington, MA
- ANMED- Rockville, MD
- Radil-University of Missouri, Columbia MO
- Virus Reference Esoterix Inc.- San Antonio, TX
- Simian Serology, BioReliance Corp., Rockville, MD
- FiloVirus-Ebola Test: Virus Simian Lab, CDC- Atlanta,
GA
- Monkey Bite Serology and Virology, Michigan State University,
East Lansing, MI
- Endocrinology and Special Enzymes, Antech Lab - NY
- What is the lab equipment?
CCMS Pathology is equipped with modern lab instruments,
which includes:
- Hematology Instrument-CDC Technology
- Chemistry Analyser-Symbiotics Inc
- Serology Instruments: ELISA reader and automatic washer-Dynex
Comp.
- Histology Processor-Sekura Comp.
- Embedding Paraffin Center-Fisher Comp.
-
Automatic Linear Stainer-Hacker Comp.
- Gene AMP PCR equipment-Perkin Elmer
- Microscopes:
- individual (light, phase-contrast, fluorescent, microphotography)
- double headed with video color camera attached to a Sony
video monitor
- dissecting microscope
The laboratory owns sets of kodachromes (over 2000) and slides
for educational purposes.
- What are the tests performed in rodents at The Center
for Laboratory Animal Sciences?
Table 2
(Z) = Zoonosis
| BACTERIOLOGY OROPHARYNX |
Mouse
|
Pig
|
G. Pig
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Hamster
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| Bordetella bronchiseptica |
X
|
X
|
X
|
X
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| Corynebacterium kutscheri |
X
|
X
|
X
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| Klebsiella pneumoniae |
X
|
X
|
X
|
X
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| Pasteurella pneumotropica |
X
|
X
|
X
|
X
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| Pseudomonas aeruginosa |
X
|
X
|
X
|
X
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| Staphylococcus aureus |
X
|
X
|
X
|
X
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| Streptococcus pneumoniae |
X
|
X
|
X
|
X
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| |
|
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| BACTERIOLOGY DIGESTIVE |
|
|
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| Salmonella spp. |
X
|
X
|
X
|
X
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| Citrobacter freundii |
X
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|
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| Campylobacter*/ Helicobacter ** |
X**
|
X*/**
|
X*
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X*/**
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| Escherichia coli |
X
|
X
|
X
|
X
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| Clostridium piliformis/ Tyzzer's disease |
X
|
X
|
X
|
X
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| |
|
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| SEROLOGY |
(Z)
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|
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| Sendai virus (SV) RNA Paramyxovirus |
X
|
X
|
X
|
X
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| Pneumonia virus of mice (PVM) RNA
Paranryxov |
X
|
X
|
X
|
X
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| Cilia-associated respiratory bacillus
(CARB) |
X
|
X
|
X
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| Reovirus 3 (Reo-3) RNA Rotavirus
Hepatoencephalom |
X
|
X
|
X
|
X
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| Cytomegalovirus (CMV) DNA Herpes |
X
|
X
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| Lymphocytic choriomeningitis v. (LCM)
Arena |
X(Z)
|
X(Z)
|
X(Z)
|
X(Z)
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| Hantaviruses (Hant) RNA Bunyaviridae |
X(Z)
|
X(Z)
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|
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| Mycoplasma pulmonis (M.pul) |
X
|
X
|
|
|
| Encephalitozoon curiiculi (E.cuniculi) |
X(Z)
|
X(Z)
|
X(Z)
|
X(Z)
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| Mouse hepatitis (MHV) RNA Coronavinus |
X
|
|
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| Mouse encephalomyelitis Theders (GD7)
RNA Pico |
X
|
X
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| Parvo virus (PV) DNA |
X
|
X
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| Ectromelia (Ectro) DNA Poxviridae |
X
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|
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| Minute virus mice (MVM) DNA Parvo |
X
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|
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| Polyomavirus (Polyoma) DNA Papovaviridae |
X
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|
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| Epidemic diarrhea infant mice (EDIM)
RNA rota |
X
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| Mouse thymic (MTV) DNA Herpes |
X
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| Papovaviridae (K virus) DNA |
X
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| Kilham rat virus (KRV) DNA Parvo |
|
X
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| Rat parvovirus-Toolan (H-1) DNA |
|
X
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| Simian virus -5 (SV5) RNA Parannyxovirus |
|
|
X
|
X
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| Parainfluenza -3 (PI-3) RNA |
|
X(Z)
|
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| Mouse adenovirus (MAD) DNA Adenovir |
X
|
X
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|
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| Sialodacryoadenitis/Corona (SDA)
RNA Coronav |
|
X
|
|
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| Clostridium piliformis -Tyzzer's (C.pil) |
X
|
X
|
X
|
X
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| PARASITOLOGY |
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|
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| Ectoparasites |
X
|
X
|
X
|
X
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| Endoparasites |
X
|
X
|
X
|
X
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| |
|
|
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| PATHOLOGY |
X
|
X
|
X
|
X
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