Iyengar Laboratory

Kenneth Bromberg, Ph.D.

Postdoctoral Fellow, Department of Pharmacology and Systems Therapeutics
E-mail: kenneth.bromberg@mssm.edu

Mailing Address

One Gustave L. Levy Place, Box 1215, New York, NY 10029

Training and Education

Ph.D. (2003) Vanderbilt University, Nashville, TN, USA

B.A. (1998) Franklin and Marshall College, Lancaster, PA, USA

Current Research

Heterotrimeric G-proteins transduce signals from cytokines, hormones and neurotransmitters at the cell surface into many intracellular responses in order to properly control cell behaviors such as growth, survival, and differentiation. Aberrant G-protein signaling is often observed in human cancers of the endocrine system, lung, ovary, prostate, and pancreas. One G-protein coupled receptor pathway implicated in cell differentiation and cancer development is the G_o pathway. Our laboratory has determined that Gα_o can trigger transformation in fibroblasts and differentiation in neurons by a conserved pathway that routes to Stat3 transcriptional activation.

Research Goals

1. Use proteomics and genomics to identify signaling pathways that are important for Gα_o-mediated neuronal differentiation
2. Integrate the experimental results with computational biology to generate a signaling network from receptor activation to gene expression
3. Experimentally validate the network

Identifying key regulatory motifs, transcriptional networks, and gene regulatory networks that are formed by these pathways will provide valuable new insight into cell differentiation and further define how these signals go awry during transformation.

Selected Publications

Bromberg KD, Iyengar R, He JC. Regulation of neurite outgrowth by Gi/o signaling pathways. Front Biosci. 2008 May 1;13:4544-57.

Bromberg KD, Ma'ayan A, Neves SR, Iyengar R. Design logic of a cannabinoid receptor signaling network that triggers neurite outgrowth. Science. 2008 May 16;320(5878):903-9.

Delaunay A, Bromberg KD, Hayashi Y, Mirabella M, Burch D, Kirkwood B, Serra C, Malicdan MC, Mizisin AP, Morosetti R, Broccolini A, Guo LT, Jones SN, Lira SA, Puri PL, Shelton GD, Ronai Z. The ER-Bound RING Finger Protein 5 (RNF5/RMA1) Causes Degenerative Myopathy in Transgenic Mice and Is Deregulated in Inclusion Body Myositis. PLoS ONE. 2008 Feb 13;3(2):e1609.

Bromberg KD, Kluger HM, Delaunay A, Abbas S, DiVito KA, Krajewski S, Ronai Z. Increased expression of the E3 ubiquitin ligase RNF5 is associated with decreased survival in breast cancer. Cancer Res. 2007 Sep 1;67(17):8172-9.

Kadoya T, Khurana A, Tcherpakov M, Bromberg KD, Didier C, Broday L, Asahara T, Bhoumik A, Ronai Z. JAMP, a Jun N-terminal kinase 1 (JNK1)-associated membrane protein, regulates duration of JNK activity. Mol Cell Biol. 2005 Oct; 25(19):8619-30.

Bromberg KD, Velez-Cruz R, Burgin AB, Osheroff N. DNA ligation catalyzed by human topoisomerase II alpha. Biochemistry. 2004 Oct 26; 43(42):13416-23.

Lindsey RH Jr, Bromberg KD, Felix CA, Osheroff N. 1,4-Benzoquinone is a topoisomerase II poison. Biochemistry. 2004 Jun 15;43(23):7563-74.

Bromberg KD, Burgin AB, Osheroff N. Quinolone action against human topoisomerase IIalpha: stimulation of enzyme-mediated double-stranded DNA cleavage. Biochemistry. 2003 Apr 1; 42(12):3393-8.

Bromberg KD, Burgin AB, Osheroff N. A two-drug model for etoposide action against human topoisomerase IIalpha. J Biol Chem. 2003 Feb 28; 278(9):7406-12.

Bromberg KD, Hendricks C, Burgin AB, Osheroff N. Human topoisomerase IIalpha possesses an intrinsic nucleic acid specificity for DNA ligation. Use of 5' covalently activated oligonucleotide substrates to study enzyme mechanism. J Biol Chem. 2002 Aug 23; 277(34):31201-6.

Bromberg KD, Osheroff N. DNA cleavage and religation by human topoisomerase II alpha at high temperature. Biochemistry. 2001 Jul 27; 40(28):8410-8.