The Mount Sinai Journal of Medicine - Mount Sinai School of Medicine

Volume 73 Number 4 July 2006	back to contents

Dean's Lecture The Use of Receptor-Specific Antibodies to Study G-Protein-Coupled Receptors	673-681
Achla Gupta, Ph.D., and Lakshmi A. Devi, Ph.D.

From the Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY.

Address all correspondence to Lakshmi A. Devi, Ph.D., Department of Pharmacology and Biological Chemistry, Box 1603, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029; e-mail: lakshmi.devi@mssm.edu

Presented to the Department of Medicine, Mount Sinai School of Medicine, New York, NY, March 13, 2004 and updated November 2005.

Abstract

The identification of G-protein-coupled receptor (GPCR) cDNAs has facilitated a number of studies characterizing the biochemical properties of the receptor protein. Most of these studies have used antibodies directed against the epitope-tagged receptor expressed in heterologous cells, because of the lack of sensitive and selective antibodies capable of recognizing endogenous receptors in their native state. In order to facilitate studies with endogenous receptors, efforts have been made to generate receptor-type selective, sensitive antibodies that are able to recognize endogenous receptors. In this review, we discuss the strategies as well as the details of the techniques used for the generation of monoclonal and polyclonal antibodies with a focus on family A GPCRs.

Key Words

Opiate, opioid receptors, morphine, monoclonal.

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