Achla Gupta, PhD
- ASSISTANT PROFESSOR | Pharmacological Sciences
Dr. Gupta is a member of the Devi Laboratory within the Department of Pharmacology and Systems Therapeutics.
MPhil, Aligarh Muslim University
PhD, Aligarh Muslim University
My research interests include the investigation of G-protein coupled receptor heterodimerization/oligomerization using antibodies as tools. I have generated an antibody against mu-delta heterodimers using subtractive immunization. This antibody recognize mu-delta heterdimers by ELISA in vitro and as well as in vivo. I have also generated selective monoclonal antibodies against the N-terminal region of opioid receptors. We have used N-terminal selective antibodies to investigate the structural changes occurring during opioid receptor activation. Interestingly, antibody recognition was found to be modulated only by agonists and not antagonists in a dose-dependent manner. The antibodies were able to distinguish agonist-mediated conformational changes between wild-type and constitutively active mutant receptors. Based on these results, I have generated conformation-sensitive polyclonal antibodies against a similar region in the N-terminus of other members of the rhodopsin family (cannabinoid and adrenergic receptors). Interestingly, these antibodies were also able to recognize active conformations of the endogenous receptors. These antibodies provide a potential tool for identifying and characterizing new compounds of therapeutic interest.
Gupta A, Mulder J, Gomes I, Rozenfeld R, Bushlin I, Ong E, Lim M, Maillet E, Junek M, Cahill CM, Harkany T, Devi LA. Increased abundance of opioid receptor heteromers after chronic morphine administration. Sci Signal 2010 Jul; 3(131): ra54.
Moron JA, Gullapalli S, Taylor C, Gupta A, Gomes I, Devi LA. Modulation of opiate-related signaling molecules in morphine-dependent conditioned behavior: conditioned place preference to morphine induces CREB phosphorylation. Neuropsychopharmacology 2010 Mar; 35(4): 955-966.