Yi Wei, PhD
- ASSISTANT PROFESSOR | Ophthalmology
BA, Northwest. Agricultural University
MS, Chinese Academy of Agricultural Sci.
PhD, Life Science College, Peking University
Mount Sinai School of Medicine
Physiological and regulatory implications of the housekeeping functions of Bacillus subtilis tetL gene
TetL is a multifunctional antiporter in Bacillus subtilis that can use a tetracycline (Tc)-divalent metal complex (with a single positive charge) or Na+ as an efflux substrate in exchange for a greater number of H+. The antiporter has physiological roles in Na+- and alkali-resistance, as well as in Tc-resistance. A mutant of B. subtilis that had a transposon insertion in the chromosomal tetL gene was Na+- and alkali-sensitive. This led to the demonstration of multiple antiport functions for TetL that are not related to its role in antibiotic-resistance. Deletion of tetL results in a spectrum of pleiotropic effects, such as Na+-, Co2+- and alkali-sensitivity as well as Tc-sensitivity. Microarray analysis of transcriptomes of such deletion strains further confirmed the irreversibility of several phenotypic features of the tetL mutants. We hypothesized that secondary mutations arise subsequent to tetL deletion that may be adaptive to stresses caused by loss of tetL. We are attempting to determine which of these secondary mutations in a tetL deletion strain might account for phenotypes such as Co2+ sensitivity. We are also trying to elucidate any possible transcriptional components around the tetL locus that might account for the Na+- and alkali-sensitive phenotype of the original mutant.\r\n
Collaborating with Dr. Bechhofer??s Lab on the tetL project\r\n
Wei Y, Guffanti AA, Ito M, Krulwich TA. Bacillus subtilis YqkI is a novel malic/Na+ lactate antiporter that enhances growth on malate at low protonmotive force. J Biol Chem. 2000 Sep 29; 275(39): 30287-30292.
Wang W, Guffanti AA, Wei Y, Ito M, Krulwich TA. Two types of Bacillus subtilis tetA(L) deletion strains reveal the physiological importance of TetA(L) in K+ acquisition as well as in Na+, alkali, and tetracycline resistance. J Bacteriol. 2000 Apr; 182(8): 2088-2095.