Shared Research Facilities (SRF)

Flow Cytometry Shared Research Facility
Icahn Medical Institute, Room 13-05
1425 Madison Avenue, Box 1090
New York, NY 10029
Tel: (212) 659-9439
Fax: (212) 803-6740

The Flow Cytometry SRF provides instrumentation and expertise for automated cytofluorimetric analysis and the sterile sorting of specific cell types. The equipment of this facility is state-of-the art; it consists of a MoFlo high speed cell sorter equipped with three lasers and a single deposition unit which allows for high speed sorting and UV analysis; a FACScan and FACS caliber analytical flow cytometers with CellQuest software, which enables the latter to perform 4-colour analysis and multi-parameter triggering for rare event analysis.

Contacts
Hans Snoeck, M.D., Ph.D., Director
Assistant Professor of Gene and Cell Medicine
Tel: (212) 659-8269
E-mail: hans.snoeck@mssm.edu

    Contact Hans Snoeck for questions concerning the planning of experiments, advice regarding analysis of data or experiments proposed in grants, or development of novel applications.

Italas George, Operator/Manager
Tel: (212) 659-9439
E-mail: italas.george@mssm.edu

    Contact Italas George for reservations, assistance with experiments, and troubleshooting with the equipment.

YanHui Deng, Operator
Tel: (212) 659-9440
E-mail: yanhui@mssm.edu

Consultation Times: BY APPOINTMENT ONLY

Location of Equipment: Icahn Medical Institute, 13th floor, Room 13-05

Equipment

Service

Operation

MoFlo high speed cell sorter (Cytomation)

Cell Sorting

This machine can only be operated by the operator and/or the director of the facility.

Reservations can be made with the operator (212) 659-9439 or ext 89439. Minimum fee of one hour is charged for equipment set-up time. Cancellations can be made 24 hours in advance; 50 percent of the hourly rate will be charged for late cancellations (less than 24 hours).


Equipment

Service

Operation

FACSVantage SE high speed cell sorter with Turbosort option and DIVA electronics (Becton Dickinson)

Cell Sorting

This machine can only be operated by the operator and/or the director of the facility.

Reservations can be made with the operator (212) 659-9439 or ext 89439. Minimum fee of one hour is charged for equipment set-up time. Cancellations can be made 24 hours in advance; 50 percent of the hourly rate will be charged for late cancellations (less than 24 hours).


Equipment

Service

Operation

FACScan and FACScalibur
with CellQuest software

Cell Analysis

This equipment is open to anyone who has taken the training session.

Those without training or who do not regularly use the equipment will be assisted by the operator. Cancellations can be made 24 hours in advance; 50 percent of the hourly rate will be charged for late cancellations.


Equipment

Service

Operation

 

Training

 

Regular users are encouraged to attend training sessions and get familiar with the equipment. This allows for use of the equipment without assistance or during off hours (less than 24 hours).


Equipment

Service

Operation

 

Use of Workstation

 

PLEASE REMEMBER: For funding purposes, it is important to acknowledge the Flow Cytometry Shared Research Facility in all publications which include data derived from the facility. Thank you.


Prices for services are available to computers on Mount Sinai networks only. For information about fees, please contact the Shared Research Facility.


Shared Research Facilities