Shared Research Facilities (SRF)

Hybridoma Shared Research Facility

Annenberg Building Room 16-05, Box 1124
One Gustave L. Levy Place
New York, NY 10029
Tel: (212) 241-6401, and (212) 241-9360
Fax: (212) 987-3653

The facility offers assistance and expertise to investigators requesting the preparation of monoclonal antibodies to a desired antigen. Currently, the Hybridoma Shared Research Facility provides a general package production of a hybridoma, which includes services such as the immunization of animals, cell fusions, screening via ELISA, isotyping, and cryopreservation. The facility also provides additional services of large scale growth of hybridoma supernatant and antibody purification. In the future, we plan to provide a wider variety of screening options such as Western blotting and flow cytometric analysis.

Contact Person

Erin Petersen, Director, Shared Research Facility
E-mail: erin.petersen@mssm.edu

Consultation Times: BY APPOINTMENT ONLY

Web Site: http://www.mssm.edu/research/resources/hybridoma/

Services

General Hybridoma Production Package

Growth of supernatant (500 ml volume)

Purification of antibody (based on 500 ml of supernatant)

Antibodies available:
* anti- c myc (9E10)

* anti- HA (12CA5)

* anti- GST

PLEASE REMEMBER -- For funding purposes, it is important to acknowledge the Hybridoma Shared Research Facility in all publications which include data derived from the facility. Thank you.

Prices for services are available to computers on Mount Sinai networks only. For information about fees, please contact the Shared Research Facility.