Shared Resource Facilities (SRF)

Quantitative PCR Shared Resource Facility
Annenberg Building Room 14-72, Box 1137
One Gustave L. Levy Place
New York , NY 10029
Tel: (212) 241-6413 or ext. 46413, and (212) 241-1710 or ext 41710
Fax: (212) 289-4107

• Mission
• Contacts
• Web Site:
  Sealfon Laboratory - Quantitative Genomics
• Introduction to Real-Time PCR
• Real-Time PCR Protocols PCR
• Publications
• Other Real-time PCR Links
• Custom Services
• Facility Equipment

Mission [top]
We aim to facilitate the effective use of quantitative real-time PCR techniques by Mount Sinai investigators. The facility provides access to high throughput robotic instruments for real-time PCR in 96-well and 384-well format, complete publication quality RNA sample-to-results custom services for real-time PCR mRNA quantification in large scale (many genes, many samples) or small scale experiments and assistance in experimental design and analysis.

Contacts [top]

For reservations, supplies, appointments:
Linford Cox
Tel: (212) 241-4226

For grant support/advice, science/technology issues, custom assays, complaints:
Stuart Sealfon, Director, Professor of Neurology
E-mail: stuart.sealfon@mssm.edu

Consultation Times: By appointment.

Web Site: Sealfon Laboratory - Quantitative Genomics

Custom Services [top]
Large-scale or small scale high quality PCR mRNA quantification is available for academic researchers on a fee-for-service basis.

Facility Equipment [top]

THE ABI PRISM 7900HT: The ABI PRISM 7900HT Sequence Detection System (SDS) is designed for high throughput detection of fluorescent PCR-related chemistries. The instrument is currently configured to use 384-well or 96-well plates and is capable of unattended 24 hour operation using a robotic arm to process plates in the queue.

The ABI 7900HT is capable of real-time, end-point, and dissociation curve analyses. A Microsoft Windows-compatible computer coordinates the Sequence Detection instrument, the bar-code reader and the automation module. The software saves the raw data from individual samples, whole plates or batches of plates for analysis. More information on the instrument can be found at Applied Biosystems' Web site at: http://home.appliedbiosystems.com.

MWG RoboGo: An 8 channel 96/384 well formal MWG pipetting robot is utilized for setting up large scale real-time PCR reactions.

Shared Resource Facilities

• Biorepository Cooperative and Histology Service
• Flow Cytometry
• Human Embryonic Stem Cell
• Hybridoma
• In-Vivo Molecular Imaging
• Microarray, PCR, Bioinformatics
• Microarray
• Microscopy
• Microvascular Surgery
• Mouse Genetics
• Mouse and Rat Phenotyping
• Quantitative PCR
• Seminar Series