High-Content Screening
Background

The High-Content Screening Shared Resource Facility (HCS-SRF) assists users in the design and execution of high-throughput experiments based on microscope images. A Molecular Devices ImageXpress Ultra confocal microscope serves as the central resource of the HCS-SRF, permitting users to collect images from cells growing in 96- or 384-well microplates. With the assistance of automated feature recognition software, user-specified features, including changes in cell shape or protein distribution, can be evaluated in thousands of distinct conditions in parallel.

This technology is particularly useful in the screening of genome-wide libraries of siRNA reagents or compound libraries. However, other approaches, including the evaluation of heterogeneity in large cell populations (image-based flow cytometry), are also possible.

For more information visit: http://compbio.mssm.edu/hcs_srf/.