Human Embryonic Stem Cell Shared Resource Facility

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Protocols

Mouse Embryonic Fibroblast Generation and Expansion

Generation of p0 Swiss-Webster Mouse Embryonic Fibroblasts (MEFs) pdf
Thawing Mouse Embryonic Fibroblasts (MEFs) pdf
Mouse Embryonic Fibroblast (MEF) Expansion/Freezing pdf

Human Embryonic Stem Cell Protocols

Human Embryonic Stem Cell (hESC) Media Constituents pdf
Thawing Human Embryonic Stem Cells (hESCs) pdf
Passaging Human Embryonic Stem Cells (hESCs) pdf
Preparing Matrigel Plates pdf
MEF Depletion/Splitting hESCs onto Matrigel pdf
Human Embryonic Stem Cell (hESC) Freezing Protocol pdf
Alkaline Phosphatase Staining pdf
Karyotyping Protocol pdf

Mouse Embryonic Stem Cell Protocols

Thawing Feeders and Mouse Embryonic Stem Cells (mESCs) Word Document
Passage 1x onto Gelatin or Feeders using DMEM-ES Media (1-2 days after thawing mESCs) Word Document
Freezing mESCs Word Document
Serum-Free mESC Differentiation Word Document
Serum Based Differentiation Media Constituents Word Document
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