Confocal Laser Scanning Microscopy

Our facility has three confocal systems to choose from. Please contact us for assistance in deciding which is best for your specific application.

Leica TCS-SP

Mated to an inverted microscope with a new Pentium 4 computer and powerful Leica LCS software, this system will excite fluorophores from UV to far red using an Ar-UV laser (350nm), an Argon laser (488nm), a Diode laser (561nm), and a HeNe laser (633nm). The spectrophotometer scan head allows the user to "tune" the detectors to any emission wavelength. The microscope can scan up to 4 channels simultaneously plus a transmitted light channel and will perform spectral separation of overlapping fluorophores.

Zeiss LSM-510 Meta

Mated to an inverted microscope with 4 lasers: a 405 nm blue diode, an Argon (458, 477, 488, 514nm), a green HeNe (543nm) and a red HeNe (633nm). The system has two PMT detectors plus META detector as well as a transmitted light detector. The META detector allows for spectral scanning of fluorescence and emission "fingerprinting" as well as unmixing of signals from fluorophores with overlapping emission profiles. This system is configured around an inverted microscope.

Zeiss Radiance 2000 MP

A multiphoton system mated to a fixed-stage upright microscope with a Ti:sapphire laser pumped by a 10W Verdi laser tunable from 720nm to 900nm and external detection system. Single photon excitation is achieved with a Krypton-Argon laser (488nm and 568nm) and a HeNe laser (647nm).