Mouse Genetics Shared Resource Facility

Protocols

Transgene Purification for Microinjection (<20kb)

Tail DNA Purification for Genotyping

ES Culture and Gene Targeting

1. Preparation of Irradiated Primary Embryo Fibroblasts
2. Electroporation and Selection of ES Cells
3. Isolation, Growth and Analysis of ES Clones
4. Preparation of ES Clones for Blastocyst Injection

Documents above marked are viewable with the free Adobe^TM Acrobat^TM Reader. If you do not have Acrobat Reader, click on the image below to download it.